| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1221 | Clostridium tertium | CTGGATGGCCACCGCATAA | AGGAACATAGCTGTTTCCCCA | 60.15 | 59.01 | 221 | 63 |
100.00%
|
100.00%
|
| Primer 1222 | Clostridium tertium | TGGATGGCCACCGCATAA | TCAACCAAGCAATTCACAGCT | 59.00 | 58.69 | 243 | 63 |
100.00%
|
100.00%
|
| Primer 1223 | Clostridium tertium | CTGGATGGCCACCGCATAA | TCAACCAAGCAATTCACAGCT | 60.15 | 58.69 | 244 | 63 |
100.00%
|
100.00%
|
| Primer 1224 | Clostridium tertium | AGGCAACATAGGGCAGCAG | GCAAGCAACCACCCACAA | 60.08 | 58.78 | 270 | 63 |
100.00%
|
100.00%
|
| Primer 1225 | Clostridium tertium | AGGCAACATAGGGCAGCA | GCAAGCAACCACCCACAA | 58.91 | 58.78 | 270 | 63 |
100.00%
|
100.00%
|
| Primer 1226 | Clostridium tertium | AAGAGACGCAATTGCAGCA | CCCCTTGCAAGCCCAGATA | 58.37 | 59.39 | 270 | 63 |
100.00%
|
100.00%
|
| Primer 1227 | Clostridium tertium | AGAGACGCAATTGCAGCAT | CCCCTTGCAAGCCCAGATA | 58.14 | 59.39 | 269 | 63 |
100.00%
|
100.00%
|
| Primer 1228 | Clostridium tertium | TGGTACTCCTGCACACACAA | CCCAATCTCCCCACCAAGT | 59.17 | 58.91 | 197 | 63 |
100.00%
|
100.00%
|
| Primer 1229 | Clostridium tertium | ATGTTGCTCATGCTTGCGG | TGATGGGCTGCCTCTTCTG | 59.49 | 59.39 | 196 | 63 |
100.00%
|
100.00%
|
| Primer 1230 | Clostridium tertium | ACCACAACACGGTCTAGCAA | ATGCAGGATGGCCACCAA | 59.53 | 59.23 | 227 | 63 |
100.00%
|
100.00%
|